Genetic Diversification of a Single Bluetongue Virus Strain Using an In Vitro Model
Christina
May 17, 2021
The Genetic Diversification of a Single Bluetongue Virus Pressure Utilizing an In Vitro Mannequin of Alternating-Host Transmission
Bluetongue virus (BTV) is an arbovirus that has been related to dramatic epizootics in each wild and residential ruminants in latest a really very long time. As a segmented, double-stranded RNA virus, BTV can evolve by a variety of mechanisms attributable to its genomic constructing. Nonetheless, the have an effect on of BTV’s alternating-host transmission cycle on the virus’s genetic diversification stays poorly understood. Full genome sequencing approaches present a platform for investigating the have an effect on of host-alternation all by all ten segments of BTV’s genome.
To know the function of alternating hosts in BTV’s genetic diversification, a subject isolate was passaged beneath three totally completely totally different circumstances: (i) serial passages in Culicoidessonorensis cells, (ii) serial passages in bovine pulmonary artery endothelial cells, or (iii) alternating passages between insect and bovine cells.
Aliquots of virus had been sequenced, and single nucleotide variants had been acknowledged. Measures of viral inhabitants genetics had been used to quantify the genetic diversification that occurred. Two consensus variants in segments 5 and 10 occurred in virus from all three circumstances.
Whereas variants arose all by all passages, measures of genetic differ remained largely comparable all by cell customized circumstances. Irrespective of passage in a relaxed in vitro system, we discovered that this BTV isolate exhibited genetic stability all by passages and circumstances. Our findings underscore the expensive function that full genome sequencing could play in enhancing understanding of viral evolution and spotlight the genetic stability of BTV.

agresearchforum
Human epithelial specific antigen,ESA ELISA Kit |
YLA2715HU-96T |
Shanghai YL Biotech |
96T |
EUR 562.5 |
Monoclonal Anti-Human Epithelial Specific Antigen (ESA/EpCAM-1/CD326) IgG |
ESA11-M |
Alpha Diagnostics |
100 ug |
EUR 578.4 |
Mouse Anti-Human Ep-CAM/Epithelial Specific Antigen monoclonal antibody, clone JID678 |
CABT-L2933-100uL500uL |
Creative Diagnostics |
100 uL, 500 uL |
EUR 602.4 |
Epithelium Specific Antigen antibody |
10R-E126A |
Fitzgerald |
50 ug |
EUR 501.6 |
Description: Mouse monoclonal Epithelium Specific Antigen antibody |
Epithelial Membrane Antigen (EMA) Antibody |
abx022470-02mg |
Abbexa |
0.2 mg |
EUR 1128 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx235427-100ug |
Abbexa |
100 ug |
EUR 661.2 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx235428-100ug |
Abbexa |
100 ug |
EUR 661.2 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx020564-1mg |
Abbexa |
1 mg |
EUR 1011.6 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx020565-1mg |
Abbexa |
1 mg |
EUR 961.2 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx020566-1mg |
Abbexa |
1 mg |
EUR 1011.6 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx023797-1mg |
Abbexa |
1 mg |
EUR 1011.6 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx020567-1mg |
Abbexa |
1 mg |
EUR 1011.6 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx020569-1mg |
Abbexa |
1 mg |
EUR 961.2 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx020571-1mg |
Abbexa |
1 mg |
EUR 961.2 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx025382-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
abx025382-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Epithelial Membrane Antigen (MUC1) Antibody |
20-abx121644 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
Epithelial Membrane Antigen (MUC1) Antibody |
20-abx123255 |
Abbexa |
|
|
|
Epithelial Membrane Antigen (MUC1) Antibody |
20-abx133246 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
Epithelial Membrane Antigen (MUC1) Antibody Pair |
abx117512-1pair5x96wellplates |
Abbexa |
1 pair (5x96 well plates) |
EUR 1212 |
|
Pig Epithelial Membrane Antigen (MUC1) ELISA Kit |
abx360587-96tests |
Abbexa |
96 tests |
EUR 990 |
|
Human Epithelial membrane antigen,EMA ELISA Kit |
201-12-1651 |
SunredBio |
96 tests |
EUR 528 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Epithelial membrane antigen,EMA ELISA Kit |
CN-04611H1 |
ChemNorm |
96T |
EUR 520.8 |
Human Epithelial membrane antigen,EMA ELISA Kit |
CN-04611H2 |
ChemNorm |
48T |
EUR 340.8 |
Human Epithelial membrane antigen(EMA)ELISA Kit |
GA-E1667HM-48T |
GenAsia Biotech |
48T |
EUR 346.8 |
Human Epithelial membrane antigen(EMA)ELISA Kit |
GA-E1667HM-96T |
GenAsia Biotech |
96T |
EUR 559.2 |
Human Epithelial membrane antigen,EMA ELISA Kit |
YLA2703HU-96T |
Shanghai YL Biotech |
96T |
EUR 562.5 |
Epithelial Membrane Antigen; Clone E29 (Concentrate) |
A00008-C |
ScyTek Laboratories |
1 ml |
EUR 596.4 |
Epithelial Membrane Antigen; Clone E29 (Concentrate) |
A00008-C.1 |
ScyTek Laboratories |
0.1 ml |
EUR 165.6 |
Sheep Epithelial Membrane Antigen (MUC1) ELISA Kit |
abx364446-96tests |
Abbexa |
96 tests |
EUR 1111.2 |
|
ELISA kit for Human Epithelial membrane antigen (EMA) |
KTE61465-48T |
Abbkine |
48T |
EUR 398.4 |
|
Description: Quantitative sandwich ELISA for measuring Human Epithelial membrane antigen (EMA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Epithelial membrane antigen (EMA) |
KTE61465-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2538 |
|
Description: Quantitative sandwich ELISA for measuring Human Epithelial membrane antigen (EMA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Epithelial membrane antigen (EMA) |
KTE61465-96T |
Abbkine |
96T |
EUR 646.8 |
|
Description: Quantitative sandwich ELISA for measuring Human Epithelial membrane antigen (EMA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Epithelial Membrane Antigen; Clone E29 (Ready-To-Use) |
A00008-0002 |
ScyTek Laboratories |
2 ml |
EUR 94.8 |
Epithelial Membrane Antigen; Clone E29 (Ready-To-Use) |
A00008-0007 |
ScyTek Laboratories |
7 ml |
EUR 129.6 |
Epithelial Membrane Antigen; Clone E29 (Ready-To-Use) |
A00008-0025 |
ScyTek Laboratories |
25 ml |
EUR 302.4 |
Epithelial Membrane Antigen; Clone E29 (Ready-To-Use) |
A00008-0500 |
ScyTek Laboratories |
500 ml |
EUR 4659.6 |
Six Transmembrane Epithelial Antigen of Prostate Detection Set |
PSI-1823 |
ProSci |
1 Set |
EUR 752.1 |
|
Description: The six-transmembrane epithelial antigen of prostate (STEAP) proteins are a family of metalloreductases identified as cell-surface antigens in prostate tissue. While STEAP2 - 4 are known to promote iron or copper reduction, the normal function of STEAP1 is still uncertain; unlike other members of the STEAP family, STEAP1 lacks the FNO-like reductase domain critical for activity. STEAP1 may be a potential target for cancer immunotherapy as its expression is highly increased in multiple cancer cell lines, including prostate, bladder, colon, and ovarian cancers and STEAP1 peptides can be used to stimulate CD8+ T cells from healthy donors, enabling them to recognize STEAP1-positive human tumor cells. STEAP2 expression in transfected cells also correlated with iron or copper uptake, suggesting that the STEAP family of proteins may function to stimulate iron and copper uptake. STEAP2 is widely expressed in many tissues in the plasma membrane, but is most highly expressed in prostate. STEAP3 is highly expressed in hematopoietic tissues and co-localizes with the transferrin endosome. Overexpression of STEAP3 stimulates iron reduction; mice lacking STEAP3 are deficient in erythroid ferrireductase activity, suggesting that STEAP3 is an endosomal ferrireductase required for transferrin-dependent iron uptake in erythroid cells. STEAP4 is highly expressed in placenta, lung, heart and prostate tissues, and overexpressed in prostate cancer cells compared to normal prostate cells. Overexpression of STEAP4 in prostate cells significantly increases cell growth and colony formation, suggesting STEAP4 may have a role in cell proliferation and prostate cancer progression. Multiple isoforms of the STEAP proteins are known to exist.;;For images please see PDF data sheet |
Six Transmembrane Epithelial Antigen of The Prostate 2 (STEAP2) Antibody |
20-abx115582 |
Abbexa |
|
|
|
Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) Antibody |
20-abx325310 |
Abbexa |
|
|
|
Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) Antibody |
20-abx174563 |
Abbexa |
|
|
|
Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) Antibody |
20-abx178397 |
Abbexa |
|
|
|
Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) Antibody |
abx331303-100ul |
Abbexa |
100 ul |
EUR 510 |
|
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) Protein |
20-abx655068 |
Abbexa |
-
EUR 693.60
-
EUR 309.60
-
EUR 2064.00
-
EUR 828.00
-
EUR 510.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
20-abx153180 |
Abbexa |
-
EUR 8853.60
-
EUR 4719.60
-
EUR 1093.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2)ELISA Kit |
201-12-2564 |
SunredBio |
96 tests |
EUR 528 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) CLIA Kit |
20-abx495377 |
Abbexa |
-
EUR 9567.60
-
EUR 5095.20
-
EUR 1177.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
DLR-STEAP2-Hu-48T |
DL Develop |
48T |
EUR 620.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) in samples from tissue homogenates, cell lysates or other biological fluids. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
DLR-STEAP2-Hu-96T |
DL Develop |
96T |
EUR 807.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) in samples from tissue homogenates, cell lysates or other biological fluids. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2(STEAP2)ELISA Kit |
QY-E01924 |
Qayee Biotechnology |
96T |
EUR 433.2 |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
RD-STEAP2-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 625.2 |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
RD-STEAP2-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 867.6 |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
RDR-STEAP2-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 652.8 |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
RDR-STEAP2-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 907.2 |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
SEH122Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5677.8 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) in Tissue homogenates, cell lysates and other biological fluids. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
SEH122Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 572.76 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) in Tissue homogenates, cell lysates and other biological fluids. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
SEH122Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 766.8 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) in Tissue homogenates, cell lysates and other biological fluids. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
SEH122Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3090.6 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) in Tissue homogenates, cell lysates and other biological fluids. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
4-SEH122Hu |
Cloud-Clone |
-
EUR 5738.40
-
EUR 3031.20
-
EUR 768.00
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2)ELISA Kit |
YLA3221HU-48T |
Shanghai YL Biotech |
48T |
EUR 435 |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2)ELISA Kit |
YLA3221HU-96T |
Shanghai YL Biotech |
96T |
EUR 562.5 |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
RD-STEAP2-Hu-48T |
Reddot Biotech |
48T |
EUR 452.7 |
|
Description: tissue homogenates, cell lysates and other biological fluids. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
RD-STEAP2-Hu-96T |
Reddot Biotech |
96T |
EUR 646.7 |
|
Description: tissue homogenates, cell lysates and other biological fluids. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
RDR-STEAP2-Hu-48T |
Reddot Biotech |
48T |
EUR 475.34 |
|
Description: tissue homogenates, cell lysates and other biological fluids. |
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit |
RDR-STEAP2-Hu-96T |
Reddot Biotech |
96T |
EUR 679.04 |
|
Description: tissue homogenates, cell lysates and other biological fluids. |
Prostate Specific Antigen Std Grade Antigen |
VAng-Cr3891-1mg |
Creative Biolabs |
1 mg |
EUR 604.8 |
Description: Prostate Specific Antigen Std Grade Antigen, Host/Source: Human Seminal Fluid. The purity is detected by salt precipitation. |
ELISA kit for Human STEAP2 (Six Transmembrane Epithelial Antigen Of The Prostate 2) |
ELK3560 |
ELK Biotech |
1 plate of 96 wells |
EUR 518.4 |
|
Description: A sandwich ELISA kit for detection of Six Transmembrane Epithelial Antigen Of The Prostate 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Prostate Specific Antigen (≥90%) |
VAng-Cr3889-1mg |
Creative Biolabs |
1 mg |
EUR 1693.2 |
Description: Prostate Specific Antigen, Host/Source: Human Seminal Fluid. The purity is ≥90% by SDS-PAGE. |
Prostate Specific Antigen (≥96%) |
VAng-Cr3890-1mg |
Creative Biolabs |
1 mg |
EUR 1693.2 |
Description: Prostate Specific Antigen, Host/Source: Human Seminal Fluid. The purity is ≥96% by SDS-PAGE. |
Anti-Rat Tumor Antigens of Epithelial Origin antibody |
STJ16100726 |
St John's Laboratory |
100 µg |
EUR 1164 |
Anti-Rat Tumour Antigens of Epithelial Origin antibody |
STJ16100727 |
St John's Laboratory |
100 µg |
EUR 1164 |
anti-Prostate Specific Antigen |
YF-PA10281 |
Abfrontier |
50 ug |
EUR 435.6 |
Description: Mouse polyclonal to Prostate Specific Antigen |
anti-Prostate Specific Antigen |
YF-PA10282 |
Abfrontier |
100 ug |
EUR 483.6 |
Description: Rabbit polyclonal to Prostate Specific Antigen |
Prostate Specific Antigen Antibody |
49127-100ul |
SAB |
100ul |
EUR 399.6 |
Prostate Specific Antigen Antibody |
49127-50ul |
SAB |
50ul |
EUR 286.8 |
Hepatocyte Specific Antigen Antibody |
V3109-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: Hepatocyte Paraffin 1 or HepPar-1 localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V3109-20UG |
NSJ Bioreagents |
20 ug |
EUR 153.3 |
Description: Hepatocyte Paraffin 1 or HepPar-1 localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V3109IHC-7ML |
NSJ Bioreagents |
7 ml |
EUR 349.3 |
Description: Hepatocyte Paraffin 1 or HepPar-1 localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V3109SAF-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: Hepatocyte Paraffin 1 or HepPar-1 localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V3131-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: Monoclonal antibodies (mAbs) to liver cell processes are useful in the identification of hepatic carcinomas and normal organ structures. mAb HSA98 binds to human hepatocytes and the majority of human hepatocellular carcinomas (HCCs). In frozen sections, it stains hepatic cells and may be used as a marker of the liver. Cell preparations of hepatocellular carcinoma biopsies or cell lines are found to bind HSA98 on the cell surface. This mAb stains liver hepatocytes in frozen human liver sections and is positive on the cell surface of human liver carcinomas. |
Hepatocyte Specific Antigen Antibody |
V3131-20UG |
NSJ Bioreagents |
20 ug |
EUR 153.3 |
Description: Monoclonal antibodies (mAbs) to liver cell processes are useful in the identification of hepatic carcinomas and normal organ structures. mAb HSA98 binds to human hepatocytes and the majority of human hepatocellular carcinomas (HCCs). In frozen sections, it stains hepatic cells and may be used as a marker of the liver. Cell preparations of hepatocellular carcinoma biopsies or cell lines are found to bind HSA98 on the cell surface. This mAb stains liver hepatocytes in frozen human liver sections and is positive on the cell surface of human liver carcinomas. |
Hepatocyte Specific Antigen Antibody |
V3131SAF-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: Monoclonal antibodies (mAbs) to liver cell processes are useful in the identification of hepatic carcinomas and normal organ structures. mAb HSA98 binds to human hepatocytes and the majority of human hepatocellular carcinomas (HCCs). In frozen sections, it stains hepatic cells and may be used as a marker of the liver. Cell preparations of hepatocellular carcinoma biopsies or cell lines are found to bind HSA98 on the cell surface. This mAb stains liver hepatocytes in frozen human liver sections and is positive on the cell surface of human liver carcinomas. |
Hepatocyte Specific Antigen Antibody |
V3173-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: mAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations. |
Hepatocyte Specific Antigen Antibody |
V3173-20UG |
NSJ Bioreagents |
20 ug |
EUR 153.3 |
Description: mAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations. |
Hepatocyte Specific Antigen Antibody |
V3173SAF-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: mAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations. |
Hepatocyte Specific Antigen Antibody |
V9119-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCCs occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V9119-20UG |
NSJ Bioreagents |
20 ug |
EUR 153.3 |
Description: Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCCs occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V9119IHC-7ML |
NSJ Bioreagents |
7 ml |
EUR 349.3 |
Description: Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCCs occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V9119SAF-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCCs occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V2341-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC's occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V2341-20UG |
NSJ Bioreagents |
20 ug |
EUR 153.3 |
Description: Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC's occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V2341IHC-7ML |
NSJ Bioreagents |
7 ml |
EUR 349.3 |
Description: Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC's occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Hepatocyte Specific Antigen Antibody |
V2341SAF-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC's occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. |
Sperm Specific Antigen 2 Antibody |
20-abx115765 |
Abbexa |
|
|
|
Erythrocyte Specific Antigen Antibody |
V2337-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: This antibody detects an erythrocyte specific antigen. The antibody shows a well defined reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. Although the exact identity of the erythrocyte specific antigen has yet to be determined, it has been shown to be distinct from Glycophorin A. This antibody is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens. |
Erythrocyte Specific Antigen Antibody |
V2337-20UG |
NSJ Bioreagents |
20 ug |
EUR 153.3 |
Description: This antibody detects an erythrocyte specific antigen. The antibody shows a well defined reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. Although the exact identity of the erythrocyte specific antigen has yet to be determined, it has been shown to be distinct from Glycophorin A. This antibody is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens. |
Erythrocyte Specific Antigen Antibody |
V2337SAF-100UG |
NSJ Bioreagents |
100 ug |
EUR 349.3 |
Description: This antibody detects an erythrocyte specific antigen. The antibody shows a well defined reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. Although the exact identity of the erythrocyte specific antigen has yet to be determined, it has been shown to be distinct from Glycophorin A. This antibody is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens. |
Anti-Prostate Specific Antigen (1B1) |
YF-MA11962 |
Abfrontier |
100 ug |
EUR 435.6 |
Description: Mouse monoclonal to Prostate Specific Antigen |
Lung Specific Antigen(LSA120) Antibody |
BNC470120-100 |
Biotium |
100uL |
EUR 238.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF647 conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC470120-500 |
Biotium |
500uL |
EUR 652.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF647 conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC550120-100 |
Biotium |
100uL |
EUR 238.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF555 conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC550120-500 |
Biotium |
500uL |
EUR 652.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF555 conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC400120-100 |
Biotium |
100uL |
EUR 238.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF640R conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC400120-500 |
Biotium |
500uL |
EUR 652.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF640R conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC430120-100 |
Biotium |
100uL |
EUR 238.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF543 conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC430120-500 |
Biotium |
500uL |
EUR 652.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF543 conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC810120-100 |
Biotium |
100uL |
EUR 238.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF680R conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC810120-500 |
Biotium |
500uL |
EUR 652.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF680R conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC610120-100 |
Biotium |
100uL |
EUR 238.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF660R conjugate, Concentration: 0.1mg/mL |
Lung Specific Antigen(LSA120) Antibody |
BNC610120-500 |
Biotium |
500uL |
EUR 652.8 |
Description: Primary antibody against Lung Specific Antigen(LSA120), CF660R conjugate, Concentration: 0.1mg/mL |
Novel Gene Rearrangement and the Full Mitochondrial Genome of Cynoglossusmonopus: Insights into the Envolution of the Household Cynoglossidae (Pleuronectiformes)
Cynoglossusmonopus, a small benthic fish, belongs to the Cynoglossidae, Pleuronectiformes. It was not normally studied attributable to its low abundance and cryptical life-style. With the intention to know the mitochondrial genome and the phylogeny in Cynoglossidae, all of the mitogenome of C. monopus has been sequenced and analyzed for the primary time. The entire dimension is 16,425 bp, usually containing 37 genes with novel gene rearrangements. The tRNA-Gln gene is inverted from the sunshine to the heavy strand and translocated from the downstream of tRNA-Ile gene to its upstream. The administration house (CR) translocated downstream to the three’-end of ND1 gene adjoining to inverted to tRNA-Gln and left a 24 bp hint fragment inside the real place.
The phylogenetic timber had been reconstructed by Bayesian inference (BI) and most chance (ML) strategies based completely on the mitogenomic data of 32 tonguefish species and two outgroups. The outcomes help the concept Cynoglossidae is a monophyletic group and stage out that C. monopus has the closest phylogenetic relationship with C. puncticeps.
By combining fossil data and mitogenome data, the time-calibrated evolutionary tree of households Cynoglossidae and Soleidae was firstly offered, and it was indicated that Cynoglossidae and Soleidae had been differentiated from one another all by Paleogene, and the evolutionary technique of household Cynoglossidae coated the Quaternary, Neogene and Paleogene durations.
Genome-Broad Identification and Characterization of the SHI-Associated Sequence Gene Household in Rice
Rice (Oryzasativa) yield is correlated to varied components. Transcription regulators are essential components, akin to the frequently SHORT INTERNODES-related sequences (SRSs), which encode proteins with single zinc finger motifs. Nonetheless, data concerning the evolutionary and wise traits of the SRS gene household members in rice is inadequate.
Subsequently, we carried out a genome-wide screening and characterization of the OsSRS gene household in Oryzasativa japonica rice. We furthermore examined the SRS proteins from 11 rice sub-species, consisting of three cultivars, 6 wild varieties, and some completely totally different genome sorts. SRS members from maize, sorghum, Brachypodiumdistachyon, and Arabidopsis had been furthermore investigated.
All these SRS proteins exhibited species-specific traits, together with monocot- and dicot-specific traits, as assessed by phylogenetic evaluation, which was additional validated by gene constructing and motif analyses. Genome comparisons revealed that segmental duplications may have carried out crucial roles contained in the recombination of the OsSRS gene household and their expression ranges. The household was primarily subjected to purifying selective stress.
Along with, the expression data demonstrated the distinct responses of OsSRS genes to varied abiotic stresses and hormonal cures, indicating their wise divergence. Our research offers an exquisite reference for elucidating the choices of SRS genes in rice.
A preliminary analysis of a domestically produced biotinylated polyclonal anti-rabies antibody for direct speedy immunohistochemical check (DRIT) within the Philippines
- Rabies is a deadly zoonotic illness endemic in growing international locations of Asia and Africa. Lately, the direct speedy immunohistochemical check (DRIT) was advisable by the World Well being Group (WHO) and the World Group for Animal Well being (OIE) as a diagnostic check for rabies. Subsequently, a biotinylated polyclonal antibody (pAb) towards the rabies lyssavirus (RABV) nucleoprotein was developed utilizing a plasmid cDNA vaccine derived from a problem virus commonplace 11 pressure.
- A preliminary analysis on the efficacy of this reagent in recognizing the Philippine RABV pressure was examined utilizing banked canine hippocampal tissue samples with DRIT and the outcomes have been in comparison with dFAT. The consequences of acetone and formalin fixation on DRIT have been additionally assessed by immunoreactivity scores of the specimens.
- Of the 142 samples examined, 104 examined optimistic and 38 detrimental utilizing each dFAT and DRIT, exhibiting 100% settlement between the 2 diagnostic procedures. Furthermore, no false optimistic or false detrimental outcomes have been noticed utilizing acetone and formalin fixation. Thus, domestically ready biotinylated pAb from plasmid cDNA can be utilized for DRIT, particularly in resource-limited laboratories within the Philippines.
- Nevertheless, these outcomes must be confirmed with a extra thorough analysis of this system, and the vary of detection must be additional evaluated in a bigger panel of animal samples and on different lyssaviruses.